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Group: Non-Oncological Applications

Other EP43 publications IRE: Exploratory literature7 publications

Nanosecond Pulsed Electric Field (nsPEF)

This heading gathers records using pulses on the nanosecond scale, thousands of times shorter than microsecond pulses and at far higher field strengths. At this scale the pulse acts on intracellular membranes rather than the plasma membrane; the themes reported in the literature are permeabilisation of the mitochondrial membrane, calcium release, caspase-dependent and caspase-independent cell death pathways, and the immune response following ablation in tumour models. Most records in the corpus are cell culture and animal studies, alongside measurement papers on pulse generators and exposure systems. The area falls outside the electrochemotherapy and irreversible electroporation scope of the portal and does not appear in the indication directory: nanosecond pulsing is a separate technology line, applied neither for drug delivery nor within standard IRE protocols. It is kept in the corpus to show how far the same biophysical family has been taken. The number of publications here does not indicate that a treatment is approved or in clinical use.

61total publications
2clinical publications
50preclinical publications
9reviews
2003–2026publication span

Clinical publications: randomized, prospective and retrospective studies, case series and case reports. Preclinical publications: animal, in vitro, modelling and experimental biophysics studies.

Literature Status by Method

Publication count, clinical and preclinical split, literature maturity and publication span for each clinical method. The badges at the top of the page summarise the Other EP and IRE records separately; the full split by method is here.

Electrochemotherapy (ECT)

4publications
0clinical publications
4preclinical publications

Exploratory literaturethreshold 0/4

No record for this method entered the maturity calculation; the records are marked in the validation ledger as conflicting, insufficient or out of scope.

2023–2026

Irreversible electroporation (IRE)

7publications
0clinical publications
6preclinical publications

Exploratory literaturethreshold 0/7

No record for this method entered the maturity calculation; the records are marked in the validation ledger as conflicting, insufficient or out of scope.

2011–2026

Pulsed-field ablation (PFA)

1publication
0clinical publications
1preclinical publication

Exploratory literaturethreshold 0/1

No record for this method entered the maturity calculation; the records are marked in the validation ledger as conflicting, insufficient or out of scope.

2024–2024

Calcium electroporation

1publication
0clinical publications
1preclinical publication

Exploratory literaturethreshold 0/1

No record for this method entered the maturity calculation; the records are marked in the validation ledger as conflicting, insufficient or out of scope.

2021–2021

Gene electrotransfer

2publications
0clinical publications
1preclinical publication

Exploratory literaturethreshold 0/2

No record for this method entered the maturity calculation; the records are marked in the validation ledger as conflicting, insufficient or out of scope.

2008–2013

Other electroporation applications

43publications
2clinical publications
36preclinical publications

This set does not name a single clinical method; no literature maturity is assigned.

2003–2026

Records with no determined method

3publications
0clinical publications
1preclinical publication

This set does not name a single clinical method; no literature maturity is assigned.

2017–2018

Mechanisms Mentioned in Abstracts

Mechanism names are detected by automatic matching over publication abstracts; the figure is the number of publications containing the name. A mention does not mean the mechanism was demonstrated in that study.

Nanosecond Pulsed Electric Field (nsPEF) 38 Apoptosis Induction 24 Reversible Membrane Permeabilisation 21 Optimisation of Pulse Parameters 19 Irreversible Pore Formation and Cell Death 10 Mitochondrial Dysfunction and Oxidative Stress 9 Disruption of Calcium Homeostasis 8 Transmembrane Potential Change 5 Thermal Effect and Joule Heating 5 Increased Intracellular Bleomycin Accumulation 4 Necrosis and Necroptosis 4 Muscle Contraction and Anaesthesia Requirement 4

Study Type Distribution

Bar length is scaled to the most common study type; the percentage gives the share of the whole area. Colour marks the study-type band, on the same scale as the literature map.

Study typePublicationsShare within this area
Prospective Clinical Study1
2%
Clinical Study1
2%
Review9
15%
Animal Study31
51%
In Vitro Study16
26%
In Silico / Modelling1
2%
Experimental Biophysics Study2
3%

Literature Evidence Table

61 publications listed in order of evidence strength. Click a title to open the citation, mechanism and full abstract drawer.

The study type, model and mechanism columns are assigned automatically and can be wrong for individual records; the abstract column is taken either from the publication's own abstract or from the opening text of the source PDF. The labels are there for scanning and filtering, not as record-level verified data; consult the full text before acting on any single publication. The classification rules and known errors are on the Literature Map.

61 publications shown

RegimePublicationYearStudy typeModelMechanismAbstract
Other EP
Li et al · J Clin Endocrinol Metab
2026Prospective Clinical StudyHumanNanosecond Pulsed Electric Field (nsPEF), Reversible Membrane PermeabilisationContext Nanosecond pulsed electric field (nsPEF) ablation has gradually been applied in clinical practice. However, no studies have reported its application in low-risk papillary thyroid mic…
Other EP
Xu et al · Int J Surg
2025Clinical StudyHumanNanosecond Pulsed Electric Field (nsPEF), Irreversible Pore Formation and Cell DeathBackground: Preclinical studies have shown that nanosecond pulsed electric field ablation (nsPEF) is a novel nonthermal ablation modality that can eradicate tumors near critical structures. …
Other EP
Zhou et al · Bioelectrochemistry
2023ReviewHumanNanosecond Pulsed Electric Field (nsPEF), Thermal Effect and Joule HeatingBackground: Nanosecond pulsed electric field, with its unique bioelectric effect, has shown broad application potential in the field of tumor therapy, especially in malignant tumors and skin…
Other EP
Pakhomov et al · Bioelectrochemistry
2020ReviewAnimal (In Vivo)Reversible Membrane Permeabilisation, Transmembrane Potential ChangeConventional electric stimuli of micro- and millisecond duration excite or activate cells at voltages 10-100 times below the electroporation threshold. This ratio is remarkably different for…
Undetermined
Beebe · Handbook of Electroporation
2017ReviewLiterature AnalysisNanosecond Pulsed Electric Field (nsPEF)Nanosecond pulses have been used for over a decade to treat tumors in preclinical studies in mice and rats. The technology based on pulsed power refocuses cancer treatment from chemistry and…
Other EP
Beebe · Handbook of Electroporation
2017ReviewLiterature AnalysisApoptosis Induction, Nanosecond Pulsed Electric Field (nsPEF)This chapter will focus on how nanosecond pulsed electric fields (nsPEFs) induce apoptosis and regulated cell death (RCD) in cells in vitro. When studies analyzing nsPEF-induced cell death (C…
Undetermined
Leveque et al · Handbook of Electroporation
2017ReviewLiterature AnalysisThis chapter summarizes the experimental dosimetry techniques developed for exposures to high-intensity, ultrashort pulses in the nanosecond domain. The dosimetry or characterization of the …
Other EP
Mi et al · Handbook of Electroporation
2017ReviewLiterature AnalysisNanosecond Pulsed Electric Field (nsPEF)In order to explore the biomedical application of nanosecond pulsed electric fields (nsPEFs), researchers have used numerous methods to develop nanosecond pulse generators. Transmission line-…
Other EP
Nuccitelli · Handbook of Electroporation
2017ReviewLiterature AnalysisApoptosis InductionNanosecond electric pulses were first applied to mice for tumor ablation in 2002. Since that time this technology has been used in dozens of laboratories in the USA, Europe, and Asia (Batista…
IRE
Breton et al · Bioelectromagnetics
2011ReviewHumanNanosecond Pulsed Electric Field (nsPEF), Irreversible Pore Formation and Cell DeathNew local treatments based on electromagnetic fields have been developed as non-surgical and minimally invasive treatments of tumors. In particular, short electric pulses can induce importan…
Gene ET
Sundararajan · Mol Biotechnol
2008ReviewHumanGene Electrotransfer (Plasmid DNA)As the medical field moves from treatment of diseases with drugs to treatment with genes, safe and efficient gene delivery systems are needed to make this transition. One such safe, non-vira…
IRE
Guo · PLoS One
2026Animal StudyAnimal (In Vivo)Nanosecond Pulsed Electric Field (nsPEF), Increased Intracellular Cisplatin AccumulationNanosecond Pulsed Electric Field (nsPEF) is an intense electrical pulse technology with ultra-short duration (nanoseconds), capable of generating electric field intensities up to several kV/…
IRE
Sano et al · IEEE Trans Biomed Eng
2026Animal StudyAnimal (In Vivo)Reversible Membrane Permeabilisation, Irreversible Pore Formation and Cell DeathObjective: The objective of this study was to investigate the effect of electrical dose on in vivo INSPIRE treatments which administer high voltage ultrashort alternating polarity electrical…
IRE
Fong · Cancers (Basel)
2025Animal StudyAnimal (In Vivo)Irreversible Pore Formation and Cell Death, Reversible Membrane PermeabilisationObjectives: There is a critical need for effective focal therapies for patients with inoperable or anatomically complex tumors where conventional ablation techniques pose high risk or are in…
ECT
Malakauskaitė et al · Sci Rep
2025Animal StudyAnimal (In Vivo)Mitochondrial Dysfunction and Oxidative Stress, Reversible Membrane PermeabilisationElectroporation can be successfully employed for controlled molecular delivery and therefore has found clinical applications for treatment of cancer. However, it’s a pulse-dependent phenomen…
IRE
Williamson et al · Bioelectricity
2025Animal StudyAnimal (In Vivo)Irreversible Pore Formation and Cell Death, Reversible Membrane PermeabilisationObjective: To study the safety and reproducibility of high-voltage integrated nanosecond pulse irreversible electroporation (INSPIRE) administered through a single applicator and grounding p…
PFA
Nuccitelli et al · Bioelectricity
2024Animal StudyAnimal (In Vivo)Irreversible Pore Formation and Cell Death, Necrosis and NecroptosisThe purpose of this study was to demonstrate the safety and performance of the CellFX Percutaneous Electrode for delivering nanosecond pulsed field ablation (nsPFA) energy to soft tissues. T…
Other EP
Gudvangen et al · Sci Rep
2022Animal StudyAnimal (In Vivo)Muscle Contraction and Anaesthesia Requirement, Nanosecond Pulsed Electric Field (nsPEF)Ablation therapies aim at eradication of tumors with minimal impact on surrounding healthy tissues. Conventional pulsed electric field (PEF) treatments cause pain and muscle contractions far…
Other EP
Kim et al · Int J Mol Sci
2021Animal StudyAnimal (In Vivo)Nanosecond Pulsed Electric Field (nsPEF), Muscle Contraction and Anaesthesia RequirementIntense pulsed electric fields (PEF) are a novel modality for the efficient and targeted ablation of tumors by electroporation. The major adverse side effects of PEF therapies are strong inv…
Other EP
Bagalkot et al · Sci Rep
2019Animal StudyAnimal (In Vivo)Reversible Membrane Permeabilisation, Nanosecond Pulsed Electric Field (nsPEF)Exposing adrenal chromaffin cells to single 150 to 400 ns electric pulses triggers a rise in intracellular Ca 2+ ([Ca 2+ ] i ) that is due to Ca 2+ influx through voltage-gated Ca 2+ channel…
Other EP
Casciola et al · Cell Mol Life Sci
2019Animal StudyAnimal (In Vivo)Optimisation of Pulse ParametersThe initiation of action potentials (APs) by membrane depolarization occurs after a brief vulnerability period, during which excitation can be abolished by the reversal of the stimulus polar…
Other EP
Rossi et al · Sci Rep
2019Animal StudyAnimal (In Vivo)Nanosecond Pulsed Electric Field (nsPEF), Apoptosis InductionAccumulating data indicates that some cancer treatments can restore anticancer immunosurveillance through the induction of tumor immunogenic cell death (ICD). Nanosecond pulsed electric fiel…
Other EP
Rossi et al · Cancers (Basel)
2019Animal StudyAnimal (In Vivo)Nanosecond Pulsed Electric Field (nsPEF), Immunogenic Cell Death (ICD)Depending on the initiating stimulus, cancer cell death can be immunogenic or non-immunogenic. Inducers of immunogenic cell death (ICD) rely on endoplasmic reticulum (ER) stress for the traf…
Undetermined
Lassiter et al · Cancers (Basel)
2018Animal StudyAnimal (In Vivo)Nanosecond Pulsed Electric Field (nsPEF), Apoptosis InductionNano-pulse stimulation (NPS), previously called nsPEFs, induced a vaccine-like effect after ablation of orthotopic N1-S1 hepatocellular carcinoma (HCC), protecting rats from subsequent chall…
Other EP
Ruzgys et al · Sci Rep
2018Animal StudyAnimal (In Vivo)Gene Electrotransfer (Plasmid DNA), Optimisation of Pulse ParametersCurrent electrotransfection protocols are well-established for decades and, as a rule, employ long micro-millisecond range electric field pulses to facilitate DNA transfer while application …
Other EP
Semenov et al · Bioelectrochemistry
2018Animal StudyAnimal (In Vivo)Reversible Membrane PermeabilisationDecreasing the time gap between two identical electric pulses is expected to render bioeffects similar to those of a single pulse of equivalent total duration. In this study, we show that it…
Other EP
Gianulis et al · Arch Biochem Biophys
2015Animal StudyAnimal (In Vivo)Lanthanide ions are the only known blockers of permeabilization by electric pulses of nanosecond duration (nsEP), but the underlying mechanisms are unknown. We employed timed applications of…
Other EP
Nuccitelli et al · PLoS One
2015Animal StudyAnimal (In Vivo)Nanosecond Pulsed Electric Field (nsPEF), Immunogenic Cell Death (ICD)We have used both a rat orthotopic hepatocellular carcinoma model and a mouse allograft tumor model to study liver tumor ablation with nanosecond pulsed electric fields (nsPEF). We confirm t…
Other EP
Silve et al · Bioelectrochemistry
2015Animal StudyAnimal (In Vivo)Tissue Conductivity and Impedance Change, Reversible Membrane PermeabilisationElectric pulses of a few nanoseconds in duration can induce reversible permeabilization of cell membrane and cell death. Whether these effects are caused by ionic or purely dielectric phenom…
Other EP
Vernier et al · J Membr Biol
2015Animal StudyAnimal (In Vivo)Reversible Membrane PermeabilisationNon-thermal probing and stimulation with subnanosecond electric pulses and terahertz electromagnetic radiation may lead to new, minimally invasive diagnostic and therapeutic procedures and t…
Other EP
Ibey et al · Biochem Biophys Res Commun
2014Animal StudyAnimal (In Vivo)Nanosecond Pulsed Electric Field (nsPEF), Reversible Membrane PermeabilisationMultiple studies have shown that bipolar (BP) electric pulses in the microsecond range are more effective at permeabilizing cells while maintaining similar cell survival rates as compared to…
Gene ET
Chopinet · J Membr Biol
2013Animal StudyAnimal (In Vivo)Gene Electrotransfer (Plasmid DNA)Gene electrotransfection using micro- or millisecond electric pulses is a well-established method for safe gene transfer. For efficient transfection, plasmid DNA has to reach the nucleus. Sh…
Other EP
Semenov et al · Biochim Biophys Acta
2013Animal StudyAnimal (In Vivo)Nanosecond Pulsed Electric Field (nsPEF), Disruption of Calcium HomeostasisPermeabilization of cell membranous structures by nanosecond pulsed electric field (nsPEF) triggers transient rise of cytosolic Ca(2+) concentration ([Ca(2+)](i)), which determines multifari…
Other EP
Nuccitelli et al · Int J Cancer
2012Animal StudyAnimal (In Vivo)Apoptosis Induction, Optimisation of Pulse ParametersWe have identified an effective nanoelectroablation therapy for treating pancreatic carcinoma in a murine xenograft model. This therapy initiates apoptosis in a nonthermal manner by applying…
Other EP
Nuccitelli et al · Biochem Biophys Res Commun
2012Animal StudyAnimal (In Vivo)Nanosecond Pulsed Electric Field (nsPEF), Apoptosis InductionWhen skin tumors are exposed to non-thermal, low energy, nanosecond pulsed electric fields (nsPEF), apoptosis is initiated both in vitro and in vivo. This nanoelectroablation therapy has alr…
Other EP
Pakhomova et al · Arch Biochem Biophys
2012Animal StudyAnimal (In Vivo)Nanosecond Pulsed Electric Field (nsPEF), Mitochondrial Dysfunction and Oxidative StressNanosecond pulsed electric field (nsPEF) is a novel modality for permeabilization of membranous structures and intracellular delivery of xenobiotics. We hypothesized that oxidative effects o…
Other EP
Silve et al · Bioelectrochemistry
2012Animal StudyAnimal (In Vivo)Increased Intracellular Bleomycin Accumulation, Reversible Membrane PermeabilisationIn our study, we used bleomycin to evaluate the permeabilization caused by nanosecond duration electric pulses (nanopulses). Bleomycin is a non permeant molecule which can be used both as a …
Other EP
Yin et al · PLoS One
2012Animal StudyAnimal (In Vivo)Nanosecond Pulsed Electric Field (nsPEF), Optimisation of Pulse ParametersNanosecond pulsed electric fields (nsPEF) induce apoptotic pathways in human cancer cells. The potential therapeutic effective of nsPEF has been reported in cell lines and in xenograft anima…
Other EP
Nuccitelli et al · Biochem Biophys Res Commun
2006Animal StudyAnimal (In Vivo)Nanosecond Pulsed Electric Field (nsPEF)We have discovered a new, drug-free therapy for treating solid skin tumors. Pulsed electric fields greater than 20 kV/cm with rise times of 30 ns and durations of 300 ns penetrate into the i…
Other EP
Beebe et al · Physiol Meas
2004Animal StudyAnimal (In Vivo)Nanosecond Pulsed Electric Field (nsPEF), Apoptosis InductionThese studies describe the effects of nanosecond (10-300 ns) pulsed electric fields (nsPEF) on mammalian cell structure and function. As the pulse durations decrease, effects on the plasma m…
Other EP
Beebe et al · DNA Cell Biol
2003Animal StudyAnimal (In Vivo)Nanosecond Pulsed Electric Field (nsPEF), Apoptosis InductionThe application of pulsed electric fields to cells is extended to include nonthermal pulses with shorter durations (10-300 ns), higher electric fields (< or =350 kV/cm), higher power (gigawa…
Other EP
Beebe et al · FASEB J
2003Animal StudyAnimal (In Vivo)Apoptosis Induction, Nanosecond Pulsed Electric Field (nsPEF)Electroporation by using pulsed electric fields with long durations compared with the charging time of the plasma membrane can induce cell fusion or introduce xenomolecules into cells. Nanos…
ECT
Malyško et al · Int J Mol Sci
2026In Vitro StudyCell CultureNanosecond Pulsed Electric Field (nsPEF), Reversible Membrane PermeabilisationBipolar cancellation (BPC) is an efficiency-limiting phenomenon in bipolar nanosecond pulsed electric field (nsPEF) exposures, in which the second, opposite-polarity phase reduces or partial…
IRE
Ye et al · J Cardiovasc Electrophysiol
2025In Vitro StudyCell CultureElectric Field Distribution and Modelling, Reversible Membrane PermeabilisationBackground: Cardiomyocyte lines are widely used in cardiovascular research. For cardiac ablation, the factors affecting cardiomyocytes by pulsed electrical field (PEF) ablation are being inv…
ECT
Rembiałkowska et al · Int J Pharm
2023In Vitro StudyCell CultureNanosecond Pulsed Electric Field (nsPEF), Potentiation of Cytotoxic Drug PermeabilityDrug delivery using nanosecond pulsed electric fields is a new branch of electroporation-based treatments, which potentially can substitute European standard operating procedures for electro…
ECT
Rembiałkowska et al · Int J Pharm
2023In Vitro StudyCell CultureNanosecond Pulsed Electric Field (nsPEF), Increased Intracellular Bleomycin AccumulationElectrochemotherapy (ECT) involves combining anticancer drugs with electroporation, which is induced by pulsed electric fields (PEFs), while the effects vary in effectiveness based on the sp…
Other EP
Kiełbik et al · Sci Rep
2021In Vitro StudyCell CultureNanosecond Pulsed Electric Field (nsPEF), Apoptosis InductionElectroporation with pulsed electric fields show a potential to be applied as an experimental focal therapy of tumors. Sub-microsecond regime of electric pulses displays unique electrophysic…
Calcium EP
Kulbacka · Cancers (Basel)
2021In Vitro StudyCell CultureCalcium Electroporation and ATP Depletion, Optimisation of Pulse Parameters(1) Background: Calcium electroporation (CaEP) is based on the application of electrical pulses to permeabilize cells (electroporation) and allow cytotoxic doses of calcium to enter the cell…
Other EP
Zhao et al · Front Oncol
2021In Vitro StudyCell CultureNanosecond Pulsed Electric Field (nsPEF), Apoptosis InductionNanosecond pulsed electric fields (nsPEFs) have emerged as a novel and effective strategy for the non-surgical and minimally invasive removal of tumors. However, the effects of nsPEFs treatm…
Other EP
Beebe et al · Cells
2013In Vitro StudyCell CultureNanosecond Pulsed Electric Field (nsPEF), Apoptosis InductionPulse power technology using nanosecond pulsed electric fields (nsPEFs) offers a new stimulus to modulate cell functions or induce cell death for cancer cell ablation. New data and a literat…
Other EP
Nuccitelli et al · Biochem Biophys Res Commun
2013In Vitro StudyCell CultureMitochondrial Dysfunction and Oxidative Stress, Nanosecond Pulsed Electric Field (nsPEF)The cellular response to 100 ns pulsed electric fields (nsPEF) exposure includes the formation of transient nanopores in the plasma membrane and organelle membranes, an immediate increase in…
IRE
Xiao et al · Bioelectromagnetics
2013In Vitro StudyCell CultureReversible Membrane Permeabilisation, Apoptosis InductionThe goal of this study was to assess the effect of nanosecond electric pulses on HepG2 human liver cancer cells. Electric pulses with a high strength of 10 kV/cm, duration of 500 ns and freq…
Other EP
Ren et al · Biochem Biophys Res Commun
2012In Vitro StudyCell CultureApoptosis Induction, Nanosecond Pulsed Electric Field (nsPEF)NsPEF ablation induces apoptosis markers, but specific cell death pathways have not been fully defined. To identify nsPEF-activated cell death pathways, wildtype human Jurkat cells and clone…
Other EP
Wang et al · PLoS One
2012In Vitro StudyCell CultureNanosecond Pulsed Electric Field (nsPEF), Apoptosis InductionTreatment of cancer often involves uses of multiple therapeutic strategies with different mechanisms of action. In this study we investigated combinations of nanosecond pulsed electric field…
Other EP
Stacey et al · Bioelectrochemistry
2011In Vitro StudyCell CultureNanosecond Pulsed Electric Field (nsPEF)We investigated the effects of nanosecond pulsed electric fields (nsPEF) on three human cell lines and demonstrated cell shrinkage, breakdown of the cytoskeleton, nuclear membrane and chromo…
Other EP
Yang et al · Technol Cancer Res Treat
2011In Vitro StudyCell CultureReversible Membrane Permeabilisation, Apoptosis InductionPulsed electric fields with nanosecond duration and high amplitude have effects on biological subjects and bring new venue in disease diagnosis and therapy. To address this respect, we inves…
Other EP
Hu et al · Phys Rev E Stat Nonlin Soft Matter Phys
2009In Vitro StudyCell CultureTransmembrane Potential Change, Potentiation of Cytotoxic Drug PermeabilitySelf-consistent evaluations of both the transmembrane potential (TMP) and possible electroporation density across membrane of spheroidal cells in response to ultrashort, high-intensity pulse…
Other EP
Vernier et al · Biochem Biophys Res Commun
2003In Vitro StudyCell CultureApoptosis Induction, Nanosecond Pulsed Electric Field (nsPEF)We report here real-time imaging of calcium bursts in human lymphocytes exposed to nanosecond, megavolt-per-meter pulsed electric fields. Ultra-short (less than 30 ns), high-field (greater t…
Other EP
Kumar et al · Micromachines (Basel)
2023In Silico / ModellingComputational ModelTransmembrane Potential Change, Reversible Membrane PermeabilisationThis paper creates an approximate three-dimensional model for normal and cancerous cervical cells using image processing and computer-aided design (CAD) tools. The model is then exposed to l…
Other EP
Napotnik et al · Bioelectromagnetics
2011Experimental Biophysics StudyCell CultureMitochondrial Dysfunction and Oxidative Stress, Reversible Membrane PermeabilisationNanosecond, high-voltage electric pulses (nsEP) induce permeabilization of the plasma membrane and the membranes of cell organelles, leading to various responses in cells including cytochrom…
Other EP
Rogers et al · IEEE Trans. Plasma Sci.
2004Experimental Biophysics StudyExperimental SystemOptimisation of Pulse ParametersAs part of a health and safety assessment of ultrawideband sources, it was useful to determine stimulation thresholds for an electrically excitable tissue down into the low nanosecond range.…

Limitations

The counts on this page are bounded by the scope of the source PDF archive and are not a systematic review of the area's literature.

A literature maturity label reflects the number of publications, not their methodological quality: it describes how much literature exists in the area and of what kind, not how strongly the treatment is supported. It does not indicate clinical use, guideline endorsement, reimbursement coverage or regulatory status, and it is not a formal GRADE assessment. The definitions and thresholds of the four tiers are on the Literature Map.

The “clinical publications” figure on this page (2) uses the portal's single definition: primary clinical studies, case series and case reports. Systematic reviews and guidelines are not included. The count feeding the badge threshold is narrower and does not count case reports; no publication in this area enters that threshold count under any method, and the badge at the top of the page rests on the ECT count alone. The two counts are not the same and cannot stand in for one another; the full threshold table is on the Literature Map.

0 of the 61 records in this area enter the threshold count. The remaining 61 are marked in the validation ledger as conflicting, insufficient or out of scoring; they appear in the table below but affect no threshold and no badge. That share (0%) is not equal across areas: it is 13% portal-wide and ranges from 0% to 42% by area. Two areas' badges therefore cannot be compared directly; a lower badge can reflect narrower verification coverage as much as a narrower literature.

Literature maturity on this page is given separately for each clinical method. The methods present in this area are: Electrochemotherapy (ECT), Irreversible electroporation (IRE), Pulsed-field ablation (PFA), Calcium electroporation, Gene electrotransfer, Other electroporation applications, Records with no determined method. Methods may share the same biophysical basis but they are distinct treatments; evidence gathered for one method cannot be generalised to another.